IN SILICO ANALYSIS FOR DETECTION OF CRYII GENE FROM LOCAL ISOLATES OF
BACILLUS THURINGIENSIS

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UEU » Scientific Articles » Bioteknologi
Posted by [email protected] at 18/11/2020 13:45:47 • 503 Views

IN SILICO ANALYSIS FOR DETECTION OF CRYII GENE FROM LOCAL ISOLATES OF BACILLUS THURINGIENSIS

Created by :
Seprianto ( None )
Febriana Dwi Wahyuni Henny Saraswati

Subject:	BIOKTEKNOLOGI
Alt. Subject :	BIOTECHNOLOGY
Keyword:	BACILLUS THURINGIENSIS IN SILICO LOCAL ISOLATES CRYII GENE GRADIENT PCR

Description:

Bacillus thuringiensis (Bt) is a gram-positive bacteria that can produce crystal proteins and exhibit a high insecticidal activity. One of the cry proteins is cryII gene that encoding an insecticidal protein against the Lepidoptera insect, especially in the Helicoverpa armigera species, which is a corn borer. This study aimed to obtain a suitable primer candidate to amplify and characterize cryII genes from Bacillus thuringiensis local isolates. In silico analysis was carried out for the purpose of amplification cryII gene, using several software such as BLAST to search sequence cryII gene, Bioedit for sequence alignment, SnapGene for analysis of genes within the genome annotation, and Primer3Plus for online primer design via https://primer3plus.com. A pair primer was selected to represent of cryII gene with a forward primer (cry2F) 5�- ATCTGGTCTCATAGGGGCGA-3� and reverse primer (cry2R) 5�-CGAGCTGTCGTGTTGCTTTG-3� with GC content percentage 55%. The purity of DNA Bacillus thuringiensis isolates B327 and B432 using nanoquant with a mark on each isolate at 1.88 and 1.99. Detection of the cryII gene from local isolates of Bt strain B327 and B432 were successfully amplified by Polymerase Chain Reaction (PCR) gradient technique using annealing temperatures at 50 and 54�C, with DNA band size of �500 bp.

Contributor	:	Radisti Ayu Praptiwi
Date Create	:	18/11/2020
Type	:	Text
Format	:	PDF
Language	:	Indonesian
Identifier	:	UEU-Article-5_0560
Collection ID	:	5_0560

Source :
In Proceedings of the 1st International Conference on Health (ICOH 2019), pages 127-132

Relation Collection:
Fakultas Ilmu Kesehatan

Coverage :
Civitas Akademika Universitas Esa Unggul

Rights :
@2020 Perpustakaan Universitas Esa Unggul

Publication URL :
https://digilib.esaunggul.ac.id/in-silico-analysis-for-detection-of-cryii-gene-from-local-isolates-ofbacillus-thuringiensis-17312.html

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